e0771 mouse breast cancer cell line  (ATCC)

Journal: Advanced Science

Article Title: Mapping the Tissue‐of‐Origins of Mesenchymal Stromal Cells in Injury Repair

doi: 10.1002/advs.202509533

Figure Lengend Snippet: BM‐MSC‐derived myofibroblasts or CAFs were not detected in distal tissue fibrosis or tumors. A) Confocal imaging of kidney sections from normal and UUO‐induced renal fibrotic Pdgfra creER ;Sp7 dre ;R26 ZT1 mice treated with tamoxifen at 2 months old. Myofibroblasts were indicated with anti‐Col1 antibody staining. B) Confocal imaging of lung sections from normal and bleomycin‐induced pulmonary fibrotic Pdgfra creER ;Sp7 dre ;R26 ZT1 mice treated with tamoxifen at 2 months old. Myofibroblasts were indicated with anti‐Col1 antibody staining. C) Confocal imaging of liver sections from normal and CCl 4 ‐induced liver fibrotic Pdgfra creER ;Sp7 dre ;R26 ZT1 mice treated with tamoxifen at 2 months old. Myofibroblasts were indicated with anti‐Col1 antibody staining. D) Confocal imaging of liver sections from normal and DDC‐induced liver fibrotic Pdgfra creER ;Sp7 dre ;R26 ZT1 mice treated with tamoxifen at 2 months old. Myofibroblasts were indicated with anti‐Col1 antibody staining. E–H) Quantification of the percentages of DAPI + Col1 + myofibroblasts that were ZsGreen + and tdTomato + in renal fibrosis (E), pulmonary fibrosis (F), CCl 4 ‐induced liver fibrosis (G) and DDC‐induced liver fibrosis (H). n = 5 mice from 4 independent experiments. I) Confocal imaging of E0771‐induced subcutaneous tumors from Pdgfra creER ;Sp7 dre ;R26 ZT1 mice treated with tamoxifen at 2 months old. Cancer associated fibroblasts (CAFs) were indicated with anti‐Col1 antibody staining. J) Confocal imaging of normal colons and AOM/DSS‐induced colorectal cancer from Pdgfra creER ;Sp7 dre ;R26 ZT1 mice treated with tamoxifen at 2 months old. CAFs were indicated with anti‐Col1 antibody staining. K,L) Quantification of the percentages of DAPI + Col1 + CAFs that were ZsGreen + and tdTomato + in subcutaneous tumors (K) and colorectal cancer (L). n = 3 mice from 3 independent experiments.

Article Snippet: The E0771 mouse breast cancer cell line (CRL‐3461, RRID:CVCL_GR23) was obtained from the American Tissue Type Collection (ATCC) in 2018.

Techniques: Derivative Assay, Imaging, Staining

Journal: Breast Cancer Research : BCR

Article Title: Hsp47 drives obesity-associated breast cancer progression by enhancing asporin deposition in adipose tissue

doi: 10.1186/s13058-025-02076-9

Figure Lengend Snippet: Silence of Hsp47 in adipose tissue suppresses HFD-induced mammary tumor growth. ( A - B ) Tumor growth curve of EO771 xenografts in mammary glands of control and Hsp47 Adi-KO mice under HFD (A) conditions ( n = 9). ( C ) Tumor growth curve of Wnt xenografts in mammary glands of control and Hsp47 Adi-KO mice under HFD condition ( n = 4). ( D - F ) IHC staining and quantification of cell proliferation marker Ki67 and cell apoptosis marker active-Caspase3 in tumor tissue from EO771 xenograft models in control and Hsp47 Adi-KO mice under HFD condition ( n = 6). ( G - H ) SHG imaging and quantification of collagen fiber in tumor tissues from EO771 xenograft models in control and Hsp47 Adi-KO mice under HFD condition ( n = 4). ( I ) Administration of Col003 attenuated HFD-induced weight gain in mice ( n = 5). ( J ) Tumors and tumor growth curve of mouse EO771 orthotopic xenograft models in control and Col003 treated mice (HFD) ( n = 10).( K-M ) Quantification of cell proliferation marker Ki67, active-Caspase3, and collagen deposition in EO771 tumors in control and Col003 treated mice (HFD) ( n = 6).Results are presented as mean ± SEM; * p < 0.05; ** p < 0.01; independent Student’s t test. Scar bar: 100 μm

Article Snippet: Mouse breast cancer cell lines EO771 was obtained from ATCC.

Techniques: Control, Immunohistochemistry, Marker, Imaging

Journal: Breast Cancer Research : BCR

Article Title: Hsp47 drives obesity-associated breast cancer progression by enhancing asporin deposition in adipose tissue

doi: 10.1186/s13058-025-02076-9

Figure Lengend Snippet: Asporin mediates Hsp47 function in regulating obesity and obesity-associated tumor progression. ( A ) Kaplan-Meier survival analysis showing the association of asporin expression with tumor recurrence in basal-like breast cancer. ASPN: asporin( B ) The body weight growth curve of wide type and asporin KO mice (HFD) (WT group, n = 7; KO group, n = 8). ( C ) Tumor growth curve of mouse EO771 orthotopic xenograft models in control and asporin KO mice (HFD) ( n = 11). ( D - F ) IHC staining and quantification of cell proliferation marker Ki67, and active-Caspase3 inEO771 tumor tissue from control and asporin KO mice under HFD condition ( n = 6). ( G ) Masson’s Trichome staining images and quantification of collagen deposition in E0771 tumor tissues from control and asporin KO mice under HFD condition ( n = 3)( H ) The IVIS images showing treatment of asporin protein promoted breast cancer cell growth in the decellularized mammary gland from Hsp47 Adi-KO mice ( n = 3).Results are presented as mean ± SEM; * p < 0.05; ** p < 0.01; independent Student’s t test. Scar bar: 100 μm

Article Snippet: Mouse breast cancer cell lines EO771 was obtained from ATCC.

Techniques: Expressing, Control, Immunohistochemistry, Marker, Staining